The nucleotide sequence and gene organization of red
نویسنده
چکیده
Red clover necrotic mosaic virus, a member of the dianthovirus group, is characterized by a genome composed of two nonhomologous single-stranded FNAs of approximately 4.0 (RNA-1) and 1.4 kb (RNA-2). The complete nucleotide sequence of the RNA-2 has been determined. RNA-2 i s 1448 nucleotides in length with a 5 terminal m7G cap and no 3 terminal poly-A t a i l or 5 terminal VPg. An open reading frame beginning at the f i r s t initiation codon at nucleotide 80 and ending at nucleotide 1030 has been identified which can encode a polypeptide of 35 kDa. RNA-2 d i rec ts the synthesis of a 35 kDa polypeptide ±a v i t ro . SP6 and T7 t ranscr ip ts from ful l length RNA-2 cDNA clones directed the synthesis of a polypeptide with the same electrophoretic mobility as the polypeptide directed from authentic RNA-2. Clones with various 3 terminal deletions both outside and within the 35 kDa open reading frame were transcribed and translated in vitro to define the l imits of the 35 kDa open reading frame. A second, small open reading frame capable of encoding a polypeptide of 4.9 kDa was also indicated from the sequence; however, there was no evidence for a protein product of that size. RNA-2 is presumed to be monocistronic and encode a cell-to-cell movement function. A small but significant amino acid sequence homology was observed with the brome mosaic virus RKA-3a polypeptide.
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تاریخ انتشار 2005